A Gossypium BAC clone contains key repeat components distinguishing sub-genome of allotetraploidy cottons

The Diploid D Genome Cottons (Gossypium spp.) of the New World

Assembly and annotation of a BAC clone from a newly sequenced genome of Anopheles funestus

Whole genome sequencing of disease vectors such as Aedes aegypti, Anopheles gambiae, and recently Anopheles funestus are enabling scientists to identify and investigate regions involved in the genetic plasticity of these vectors in order to formulate new disease control strategies. In the current study, sequence data from a single Anopheles funestus BAC clone is examined in order to predict and...

Dynamic building of a BAC clone tiling path for the Rat Genome Sequencing Project.

CLONEPICKER is a software pipeline that integrates sequence data with BAC clone fingerprints to dynamically select a minimal overlapping clone set covering the whole genome. In the Rat Genome Sequencing Project (RGSP), a hybrid strategy of "clone by clone" and "whole genome shotgun" approaches was used to maximize the merits of both approaches. Like the "clone by clone" method, one key challeng...

Phylogeny, duplication, and intraspecific variation of Adh sequences in New World diploid cottons (Gossypium l., malvaceae).

The 13 "D-genome"cotton species are a monophyletic assemblage of morphologically diverse diploids that inhabit arid to semiarid regions in Mexico, with 1 disjunct species each in Peru and the Galapagos Islands and 1 species whose range extends northward into Arizona. While these species lack commercially significant fiber (i. e., cotton), they are important in that they represent one of the par...

Adventures in the Enormous: A 1.8 Million Clone BAC Library for the 21.7 Gb Genome of Loblolly Pine

Loblolly pine (LP; Pinus taeda L.) is the most economically important tree in the U.S. and a cornerstone species in southeastern forests. However, genomics research on LP and other conifers has lagged behind studies on flowering plants due, in part, to the large size of conifer genomes. As a means to accelerate conifer genome research, we constructed a BAC library for the LP genotype 7-56. The ...